Fig. 6: CD44 is in complex with LRP6, AXIN, and DVL after Wnt induction.
From: Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop
a Mean fluorescence of chromobodies (CB) against active β-catenin in the nucleus of DMSO, CHIR, control CM, or Wnt3a CM-treated HeLa_BC1-TagGFP2 or CD44−/−_HeLa_BC1-TagGFP2 cells 24 h after induction. Control CM vs. Wnt3a CM: Mann–Whitney U-test. DMSO vs. CHIR: Student’s t test. Error bars, ±SE. b, c CD44/LRP6, CD44/DVL, and CD44/AXIN d, e in situ PLA complexes (dots) in HeLa cells treated with Wnt3a CM for 15 and 45 min, compared to control CM-treated cells. Cells were incubated with primary antibodies against CD44 and LRP6, DVL, or AXIN. Error bars, ±SE. Scale bar: b 20 μm, d 10 μm. c, e Fold induction of average interactions per cell, compared to control CM treatment. Error bars, ±SE. Student’s t test. f, g Co-immunoprecipitation of AXIN/CD44 (f) and CD44/DVL (g) on lysates of HeLa cells induced with Wnt3a CM for the indicated time points. Student’s t test/Mann–Whitney U-test: *p < 0.05, **p < 0.01, ns not significant. n = number of analyzed cells.
