Fig. 7: CD44 interacts directly with LRP6.
From: Wnt signaling is boosted during intestinal regeneration by a CD44-positive feedback loop
a FRET-based FLIM analysis of HEK293T cells stably expressing CD44EGFP+LRP6mcherry2 or only CD44EGFP, induced with Wnt3a or ontrol CM 1 h before analysis. EGFP lifetime images according to the color scale. Scale bar: 10 μm. b EGFP lifetime distribution in the pixels of the recorded images of uninduced CD44EGFP cells or CD44EGFP+LRP6mcherry2 HEK293T cells treated with Wnt3a or Control CM (n = 10). Representative data of 1 out of 3 independent experiments. n = number of analyzed cells. c Kolmogorov–Smirnov test comparing the distributions of either the CD44EGFP and the CD44EGFP+LRP6mcherry2 cell lines or CD44EGFP+LRP6mcherry2 cell line treated with Wnt3a or Control CM. ns not significant.
