Fig. 2: Opposite fibrotic effects in circRNA_37492 inhibition & overexpression. | Cell Death & Disease

Fig. 2: Opposite fibrotic effects in circRNA_37492 inhibition & overexpression.

From: The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis

Fig. 2

BUMPT cells were pre-transfected with either scrambled siRNA or siRNA circRNA_37492, scramble plasmids, or circRNA_37492 plasmids and co-treated with or without TGF-β1 for 24 h. A Type I, III collagen and FN expression by western blot analysis. B Quantification of protein band of type I collagen, type III collagen, and FN by grayscale analysis. C Type I, III collagen and FN expression by western blot analysis. D Quantification of protein band of type I collagen, type III collagen, and FN by grayscale analysis. Quantitative data are presented as the mean ± SD (n = 6 per group). *p < 0.05, TGF-β1 or siRNA circRNA_37492 group vs. double control group, TGF-β1 or circRNA_37492 plasmids group vs. double control group; #p < 0.05, siRNA circRNA_37492 with TGF-β1 group vs. TGF-β1 group, circRNA_37492 plasmids with TGF-β1 group vs. TGF-β1 group.

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