Fig. 5: MiR-7682-3p directly target the antifibrotic Fgb. | Cell Death & Disease

Fig. 5: MiR-7682-3p directly target the antifibrotic Fgb.

From: The mmu_circRNA_37492/hsa_circ_0012138 function as potential ceRNA to attenuate obstructive renal fibrosis

Fig. 5

BUMPT cells were pre-transfected with either miR-7682-3p mimic or siRNA Fgb and co-treated with or without TGF-β1 for 24 h. A 3’UTR of Fgb mRNA contained the complementary binding region sequence of miR-7682-3p. B After co-transfection with vector of Fgb 3’ UTR WT or Fgb 3’UTR MT and miR-7682-3p mimic or scramble, the luciferase activities were detected. C Western blot analysis of Fgb. D Densitometric analysis of proteins signals. E Type I, III collagen and FN expression by western blot analysis. F Densitometric analysis of proteins signals. Quantitative data are presented as the mean ± SD (n = 6 per group). *p < 0.05, TGF-β1 or miR-7682-3p mimic group vs. control group, TGF-β1 or siRNA Fgb group vs. control group, Fgb 3’UTR WT with miR-7682-3p vs. other groups; #p < 0.05, miR-7682-3p mimic with TGF-β1 group vs. TGF-β1 group, siRNA Fgb with TGF-β1 group vs. TGF-β1 group. MT, mutant type; WT, wild type.

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