Fig. 1: PrP106-126-induced mitophagy deficiency in N2a cells.
From: PINK1-parkin-mediated neuronal mitophagy deficiency in prion disease
A Mitophagy events through time show the colocalization between the autophagosome protein LC3-II and the mitochondria (labeled with DsRed-Mito) in N2a cells treated with PrP106-126. Scale bar: 10 µm. B Comparison of the localization of LC3-II and DsRed-Mito in cells in A. C Mitophagy in N2a cells treated with PrP106-126 was characterized by the COX8-mKeima fluorescence ratio change in Figure S1A. D, E Western blots of mitophagy-related proteins undergoing PrP106-126 treatments. GAPDH was used as the loading control. The “*“ in the Western blots band indicates LC3-II. Data were mean (SD); ns not significant; *P < 0.05; **P < 0.01; ***P < 0.001. All experiments were repeated at least three times.
