Fig. 2: Diabetic glomerulopathy was aggravated in asparaginyl endopeptidase (AEP) knockout mice. | Cell Death & Disease

Fig. 2: Diabetic glomerulopathy was aggravated in asparaginyl endopeptidase (AEP) knockout mice.

From: Asparaginyl endopeptidase protects against podocyte injury in diabetic nephropathy through cleaving cofilin-1

Fig. 2

A Western blot of AEP levels in wild-type (WT) and knockout mice (AEP−/−). N = 5. B Representative images for Synaptopodin, Nephrin, and WT1 in the control and STZ-induced glomeruli. Scale bar: 25 μm. C Quantitative analyses of the number of WT1-positive cells. N = 5. D Representative transmission electron microscopy (TEM) images showing morphological changes in the podocyte foot process in different groups of mice. Scale bar: 2 μm. N = 3. E Indices for glomerular filtration barrier integrity, including glomerular basement membrane (GBM) thickness, foot process width, and the number of foot processes/μm GBM. F Periodic acid-Schiff (PAS) staining showing glomerular morphological changes. Scale bar: 25 μm. G Quantitative analyses of the percentage of mesangial matrix area. N = 5. H Urinary albumin to creatinine ratio (ACR) in different groups of mice. N = 5. ****P < 0.0001, ***P < 0.001, **P < 0.01, *P < 0.05, data were represented as mean ± SEM.

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