Fig. 5: Asparaginyl endopeptidase (AEP) cleaved cofilin-1 in vitro. | Cell Death & Disease

Fig. 5: Asparaginyl endopeptidase (AEP) cleaved cofilin-1 in vitro.

From: Asparaginyl endopeptidase protects against podocyte injury in diabetic nephropathy through cleaving cofilin-1

Fig. 5

A Western blot showing the cleavage of GST-cofilin-1 after incubation with active recombinant AEP (rAEP) at pH 7.4 or pH 6.0. B Western blot showing the cleavage of purified GST-cofilin-1 recombinant protein by active recombinant AEP at pH 6.0. C Western blot showing the cleavage of cofilin-1 by wild-type (WT) and mutant AEP. D Western blot showing the effect of AENK and AEQK on cofilin-1 cleavage.

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