Fig. 6: Asparaginyl endopeptidase (AEP) cleaved cofilin-1 at N138 in vitro and in vivo. | Cell Death & Disease

Fig. 6: Asparaginyl endopeptidase (AEP) cleaved cofilin-1 at N138 in vitro and in vivo.

From: Asparaginyl endopeptidase protects against podocyte injury in diabetic nephropathy through cleaving cofilin-1

Fig. 6

A Cleavage of purified GST-cofilin-1 analyzed by Coomassie blue staining. B Mass spectrometry analysis of recombinant cofilin-1 fragmented by AEP. The detected MS/MS peptide spectra are listed. C Cofilin-1 cleavage was analyzed by western blot after GST-cofilin-1 wide-type, N138A, were incubated with active recombinant AEP. Western blot analysis (D) and summarized data (E) showing the levels of cofilin-1 N138 cleaved fragments in kidney cortex from control mice (Ctrl) and streptozotocin (STZ)-induced diabetic nephropathy (DN) mice (STZ-DN), N = 5. F Immunohistochemical staining of Ctrl and STZ-DN glomeruli. G Colocalization of cofilin-1 N138 fragment (green) with Synaptopodin (red), in the glomeruli of both Ctrl mice and STZ-DN mice. Scale bar 25 μm. Western blot analysis (H) and summarized data (I) showing the levels of cofilin-1 N138 cleaved fragments in kidney cortex from wild-type (WT) and AEP knockout (AEP−/−) mice. N = 5. ****P < 0.0001, **P < 0.01, data were represented as mean ± SEM.

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