Fig. 7: Decreased phosphorylation level of cofilin-1 1-138 fragments and its role in cultured podocytes. | Cell Death & Disease

Fig. 7: Decreased phosphorylation level of cofilin-1 1-138 fragments and its role in cultured podocytes.

From: Asparaginyl endopeptidase protects against podocyte injury in diabetic nephropathy through cleaving cofilin-1

Fig. 7

A Representative western blot showing the phosphorylation levels of cofilin-1 at serine 3 in podocytes transfected with full-length cofilin-1 or cofilin-1 1-138 adenovirus. B Microscopic images of F-actin by rhodamine-phalloidin staining. Scale bar: 50 μm. C Summarized data from counting the cells with distinct, longitudinal F-actin fibers. Scoring was determined from 100 podocytes on each slide. N = 4. Representative western blot (D) and summarized data (E) showing the protein levels of Desmin and cleaved Caspase 3 in podocytes transfected with green fluorescent protein (GFP) adenovirus, full-length cofilin-1, or cofilin-1 1-138 adenovirus. N = 6. ***P < 0.001, *P < 0.05, n.s. no significance, data were represented as mean ± SEM.

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