Fig. 6: UBC9 modulates PDPK1 activity by mediating its SUMOylation.
From: SUMOylation of PDPK1 Is required to maintain glycolysis-dependent CD4 T-cell homeostasis
A CD4 T cells were immune-precipitated by SUMO1 and probed with PDPK1. Band shift of PDPK1 SUMOylation (Su-PDPK1, top arrow) was observed below 100KD (representative of 5 times result). B Schematic view of SUMOylation sites within protein-kinase domain and PH domain. C Triple PDPK1 mutants (K210/K299/K498) confirmed the SUMOylation sites (representative of 2-times result). D, E Series of double mutants (K210/K299, K210/K498, and K299/K498) and K299 single mutant identified K299 as the major SUMOylation site (representative of 3-times result). F, G Phospho-proteomic study identified Mu/WT downregulated phosphoproteins that are downstream of PDPK1 and related to mTORC1 signaling, as indicated by Heatmap and KEGG pathway enrichment analysis.
