Fig. 7: Cytotoxic effect of d-astrocytes on glutamatergic neurons. | Cell Death & Disease

Fig. 7: Cytotoxic effect of d-astrocytes on glutamatergic neurons.

From: Massive iron accumulation in PKAN-derived neurons and astrocytes: light on the human pathological phenotype

Fig. 7

A Representative images of d-astrocytes from control and PKAN patients stained with the iron-specific Perls reaction (blue) and counterstained with nuclear fast red. Yellow arrows indicate iron granules. B Amount of glutamate released by the d-astrocytes measured by glutamate assay kit colorimetric (ABCAM) normalized on cell total protein content. Bars indicate means + SD, n = 3 (Student’s t-test). *p < 0.05, **p < 0.01, ***p < 0.001. C Mature glutamatergic neurons and d-astrocytes were cocultured for three days, and after removal of the d-astrocytes, the glutamatergic neurons were quantified by measuring the DAPI staining using a Tecan plate reader. Bars indicate means + SD, n = 3 (Student’s t-test). *p < 0.05, **p < 0.01, ***p < 0.001.

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