Fig. 1: Expression and roles of PCLAF in neuroblastoma.
From: PCLAF promotes neuroblastoma G1/S cell cycle progression via the E2F1/PTTG1 axis
(A) Representative pictures of distinct levels (0–4) of IHC staining of PCLAF and the proportions of five levels; (B, C) 10NB, 8GNB and 8GN tumor tissue proteins extracted for Western Blot to discover PCLAF expression, and Image J was used to quantify protein bands. The data in (C) were analyzed by Mann-Whitney U-test (n = 28); (D) Kaplan-Meier analysis of OS in TMA of 70 neuroblastoma samples based on PCLAF expression with the log-rank test P value indicated; (E–H) Kaplan-Meier analysis of OS and EFS for the SEQC data set (n = 498) and the NRC data set (n = 275), based on PCLAF expression with the log-rank test p value indicated; (I–K) PCLAF expression levels in stage (St) 1-4 S tumors was show in box plot. (*p < 0.05, ***p < 0.001and ****p < 0.0001, and bar graphs represent the mean ± SEM).
