Fig. 4: RNA sequencing reveals down-regulation of PPARɤ targeted genes in preadipocytes after deletion of LXN.

A Volcano plot of differentially expressed genes (DGEs) in WT and KO preadipocytes cultured with normal medium (KO vs WT), WT preadipocytes cultured with normal medium and differentiation medium (DM_WT vs WT) and WT or KO preadipocytes cultured with differentiation medium (DM_KO vs DM_WT). B The overlapping genes identified in different experimental groups. C DEGs identified by RNA-seq were presented in Heatmap. D GO enrichment analysis of DEGs in DM_WT vs WT group (left) and DM_KO vs DM_WT group (right). E–G Heatmap shows the GSEA of representative KEGG pathway in WT preadipocytes cultured with differentiation medium or normal medium (E), WT and KO preadipocytes cultured with normal medium (F) and differentiation medium (G). H Real-time PCR results for analysis of LXN and PPARɤ targeted genes in WT or KO preadipocytes under normal medium or differentiation medium. All results were presented as mean ± SD. *P < 0.05, and **P < 0.01, ns: no significance.