Fig. 2: p38γ shRNA inhibits NPC cell progression in vitro.
From: The pro-tumorigenic activity of p38γ overexpression in nasopharyngeal carcinoma
The immortalized NPC cell lines (CNE-1, HONE-1 and CNE-2) (A–L), the primary human NPC cells (“pNPC-1”, I–L), or the primary human nasal epithelial cells (pHNEpC-1 and pHNEpC-2, derived from two donors) (M–O) were infected with lentivirus-encoded shRNA (p38γ-shRNA-s1, p38γ-shRNA-s2 or the the scramble control shRNA/shC), with stable cells established after puromycin selection, expression of listed genes was tested by qRT-PCR (A, B, I and M) and Western blotting (C) assays. Cells were further cultured for designated periods, cell viability (by recording CCK-8 intensities, D, J and N), colony formation (E) and cell proliferation (by testing the EdU-positive nuclei ratios, F, K and O) as well as cell migration (“Transwell” assays, G and L) and invasion (“Matrigel Transwell” assays, H) were tested by the indicated assays. “pare” stands for the parental control cells. *P < 0.05 vs. “shC” cells. Scale Bar = 100 μm (F, G and O).
