Fig. 4: Oxethazaine can inhibit AURKA kinase activity. | Cell Death & Disease

Fig. 4: Oxethazaine can inhibit AURKA kinase activity.

From: Oxethazaine inhibits esophageal squamous cell carcinoma proliferation and metastasis by targeting aurora kinase A

Fig. 4

A Oxethazaine suppresses AURKA kinase activity in a dose-dependent manner. B Western blotting showing the expression levels of p-Histone H3 (S10), Histone H3, and AURKA after treatment of various concentrations of oxethazaine. C, D Immunofluorescence of p-Histone H3 and AURKA in KYSE150 and KYSE450 cells treated with oxethazaine for 24 h was photographed using the IN Cell Analyzer 6000. E The quantitative analyses of fluorescence intensity in KYSE150 and KYSE450. F Western blotting showing the expression levels of p-AURKA (T288) after treatment of various concentrations of oxethazaine. G–H Immunofluorescence of p-AURKA in KYSE150 and KYSE450 cells treated with oxethazaine for 24 h was photographed using the IN Cell Analyzer 6000. I The quantitative analyses of fluorescence intensity in KYSE150 and KYSE450. All data are shown as means ± S.D. The asterisks (*, **, ***) indicate statistical significance (p < 0.05, p < 0.01, p < 0.001, respectively).

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