Fig. 5: AURKA knockout suppresses ESCC cell growth and reduces the sensitivity of ESCC cells to oxethazaine. | Cell Death & Disease

Fig. 5: AURKA knockout suppresses ESCC cell growth and reduces the sensitivity of ESCC cells to oxethazaine.

From: Oxethazaine inhibits esophageal squamous cell carcinoma proliferation and metastasis by targeting aurora kinase A

Fig. 5

A The expression of AURKA between tumor and adjacent normal tissues across all TCGA tumors using the TIMER 2.0 website. B The expression of AURKA between esophageal tumor tissues and normal tissues. Data are based on TCGA database results from the UALCAN website. C CRISPR/Cas9 was used to knockout AURKA in KYSE150 and KYSE450 cells. Knock-out efficiency in KYSE150 and KYSE450 cell lines was assessed by Western blotting. D, E Cell proliferation and colony formation assay results of KYSE150 and KYSE450 cells transfected with sgAURKA-1, sg AURKA-2, or sgControl. F The inhibitory effect of oxethazaine on AURKA knockout cells was detected by proliferation assay after 72 h. All data are shown as means ± S.D. The asterisks (*, **, ***) indicate a significant decrease (p < 0.05, p < 0.01, p < 0.001, respectively).

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