Fig. 5: The mechanism underlying Nanog-mediated Rad51 inhibition. | Cell Death & Disease

Fig. 5: The mechanism underlying Nanog-mediated Rad51 inhibition.

From: Identification of Nanog as a novel inhibitor of Rad51

Fig. 5

a HeLa cells overexpressing Nanog fragments were stained with the anti-Rad51 antibody (red) and DAPI (blue). b Rad51 foci in the cells from a were counted and analyzed. The nuclei containing more than 10 foci were considered to be positive. c, d EMSA assay showing the capability of full-length and fragments of Nanog in inhibiting Rad51 binding to ssDNA. The right panels show the results of the Coomassie brilliant blue staining of the purified Nanog fragments. The data are based on three independent repeats, and presented as mean ± SEM. **p < 0.01; *p < 0.05 (Student’s t-test).

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