Fig. 6: ZIF-8 or TAT tag-mediated direct delivery of Nanog fragments into cells achieves Rad51 inhibition. | Cell Death & Disease

Fig. 6: ZIF-8 or TAT tag-mediated direct delivery of Nanog fragments into cells achieves Rad51 inhibition.

From: Identification of Nanog as a novel inhibitor of Rad51

Fig. 6

a TEM image (the upper panel) and UV-Vis spectra (the lower panel) of Nanog fragments@ZIF-8. b Schematic illustration of the experiment procedures. c C@ZIF-8 and CD2@ZIF-8 retarded γH2AX removal. HCT116 cells were treated with CPT for 6 h. The medium was changed to fresh CPT-free medium containing B02, C@ZIF-8 and CD2@ZIF-8, respectively, and allowed cells to grow for additional 6 and 16 h. Whole cell proteins and histones were extracted and subjected to SDS-PAGE. d N@ZIF-8 failed in delaying γH2AX removal. Similar experiments with c were employed. e TAT-mediated uptake of C and CD2 into cells retarded γH2AX removal. TAT-fused Nanog fragments were added into HCT116 after 6-h CPT treatment. The cells grew for additional 6 h. Whole cell proteins and histones were extracted and subjected to SDS-PAGE. The data are based on three independent repeats.

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