Fig. 4: Silencing INHBA inhibited the growth of CRC cells.

a, b qPCR (a) and western blot (b) were used to verify the knockdown efficiency of INHBA (Student’s t test). c, d CCK8 (c), and EdU assay (d) analyzed the proliferation of LoVo cells after knocking down INHBA (Student’s t test). e Flow cytometry was used to measure cell cycle distribution. f Western blot detecting a synergistic effect of metformin and siRNA in downregulating the expression of INHBA. g CCK8 showing the synergistic effect of metformin and siRNA in the proliferation of LoVo cells. The values are shown as the mean ± SD and experiments were independently repeated three times. *P < 0.05, ***P < 0.001. The data in this figure were analyzed by two-way ANOVA.