Fig. 5: Overexpression of INHBA antagonized the antitumor effect of metformin. | Cell Death & Disease

Fig. 5: Overexpression of INHBA antagonized the antitumor effect of metformin.

From: Metformin suppresses the growth of colorectal cancer by targeting INHBA to inhibit TGF-β/PI3K/AKT signaling transduction

Fig. 5

a, b Western blot (a), and qPCR (b) was used to confirm the efficiency of INHBA overexpression. c The concentration of INHBA in a conditioned medium of 1 × 106 cells was determined by ELISA. d–f EdU (d), CCK8 (e), and flow cytometry (f) showing the promotion effect of INHBA on tumor cell growth. g Western blot analyzing the protein level of INHBA. INHBA-GFP: the exogenous fusion protein. h CCK8 analyzing the effect of metformin on cell viability after overexpression INHBA. i CCK8 detecting the influence of condition medium from the INHBA overexpression cells on metformin inhibiting SW480 cell growth. CM: condition medium from the cells overexpressing INHBA. The values are shown as the mean ± SD and experiments were independently repeated three times. *P < 0.05, ***P < 0.001. The data in this figure were analyzed by two-way ANOVA.

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