Fig. 2: Single-cell RNA-seq analysis of the granzyme failure in the cytotoxic cells from the tumor tissues of HCC patients.

A The t-SNE map depicting clusters of cytotoxic cells in the tumor tissues and adjacent tissues of eight HCC patients. B Expression heatmap of the marker genes in each cytotoxic cell cluster (top 5, color-coded according to the cluster and expression). C Expression t-SNE maps for GZMA in the cytotoxic cells of the tumor tissues and adjacent tissues. D Expression t-SNE maps for GZMA in the cytotoxic cells from the tumor tissues. E Expression t-SNE maps for GZMA in the cytotoxic cells from the adjacent tissues. F Bar plot illustrating the ratio of GZMA positive cytotoxic cells in the tumor tissues and adjacent tissues for each HCC patient.