Fig. 3: Cellular communication in cytotoxic cells and tumor cells.

A The t-SNE map depicting clusters of cytotoxic cells and tumor cells in the tumor tissues and adjacent tissues of eight HCC patients. B The t-SNE map depicting the types of cytotoxic cells and tumor cells in the tumor and adjacent tissues, tumor tissues, and adjacent tissues of HCC patients, respectively. C Network depicting the PAR signaling pathway in cytotoxic cells and tumor cells. Color indicates the significance of clusters in the PAR signaling pathway. D Circle plot illustrating the PAR signaling pathway in cytotoxic cells and tumor cells. The color and line indicate the significance of clusters in the PARs signaling pathway. E Bar plot illustrating the contribution of ligands and receptors in the PAR signaling pathway in cytotoxic cells and tumor cells. F Fluorescence confocal assay results demonstrating the co-localization of F2R (green) and GZMA (red) in cultured cells (left) and tissues of an HCC patient (right). The nuclear DNA was stained with DAPI (blue). Scale bars: 15 μm. G Expression t-SNE maps for F2R in the tumor tissues and adjacent tissues, tumor tissues, and adjacent tissues of eight HCC patients.