Fig. 5: The molecular mechanism underlying GZMA and F2R expressions in tumor suppression. | Cell Death & Disease

Fig. 5: The molecular mechanism underlying GZMA and F2R expressions in tumor suppression.

From: Heterogeneity induced GZMA-F2R communication inefficient impairs antitumor immunotherapy of PD-1 mAb through JAK2/STAT1 signal suppression in hepatocellular carcinoma

Fig. 5

A Differentially Expressed Genes between high GZMA/F2R expression and low GZMA/F2R expression tissues in the LIHC from TCGA, identified using the gene set enrichment analysis (GSEA). B Western blots indicating the expressions of the JAK2, p-JAK2, F2R, and GZMA in the above-stated co-cultured cells. C Nuclear extraction assay. The nuclear protein and the cytoplasm protein in the above-stated cells were extracted using the nuclear extraction kit. Western blots indicated the level of STAT1 in the nucleus and the cytoplasm. D Fluorescence confocal assay results illustrating the subcellular localization of STAT1 (red) in the above-stated co-cultured cells. The nuclear DNA was stained with DAPI (blue). Scale bars: 15 μm.

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