Fig. 7: Functional role of GZMA and F2R expressions in the antitumor efficacy of PD-1 mAb therapy. | Cell Death & Disease

Fig. 7: Functional role of GZMA and F2R expressions in the antitumor efficacy of PD-1 mAb therapy.

From: Heterogeneity induced GZMA-F2R communication inefficient impairs antitumor immunotherapy of PD-1 mAb through JAK2/STAT1 signal suppression in hepatocellular carcinoma

Fig. 7

A, B The C57BL/6 mice were injected with 5 × 105 F2R-Lv-infected Hepa1-6 cells and then subjected to PD-1 mAb treatment or receiving IgG2a isotype control. A A schematic representation of the treatment plan for immune-competent C57BL/6 mice. B Plots of Hepa1-6 tumor volumes, which were recorded once a week. C, D BALB/c nude mice, injected with GZMA-rAd or RFP-rAd-infected CD3+ T cells, were injected with 2 × 105 F2R-Lv-infected Huh7 cells and then subjected to PD-1 mAb treatment or receiving the IgG2a isotype control. C A schematic representation of the treatment plan for immune-deficient BALB/c nude mice. D Plots of tumor volumes, which were recorded once a week. E Representative images of the immunohistochemistry staining of GZMA and F2R expressions in the tumor samples from HCC patients. Scale bars: 50 mm. F The densities of GZMA and F2R in PD-1 mAb responder and non-responder HCC patients. G Tumor diameters recorded by a radiologist based on CT imaging, indicated with a red line. H The changed tumor diameter (mm) in the HCC patients treated with PD-1 mAb. The tumors were with an increased diameter are indicated in red, while the tumors with a decreased diameter are indicated in green. I Spearman’s rank correlation analysis was performed to determine the quantitative correlation between the tumor diameter change and the GZMA and F2R expression levels. The results were expressed as mean ± SEM; *p < 0.05, *p < 0.01, ***p < 0.001, ****p < 0.0001.

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