Fig. 5: Leptin influences LSCs through DHH signaling.

Leptin augments LSCs through DHH signaling. Cells (LSCs, ALCs, Sertoli cells, PMCs) were treated with Vismodigib (DHH antagonist) in the presence of Leptin (1 or 10 ng/ml). Immunostaining showing expression of A alpha SMA, B SMHC, C Vimentin, and D B3HSD upon post-Vismodigib and leptin treatment. E Quantification of the experimental data to compare the effects of treatment. Asterisks denote p values (e.g., *p < 0.05, ****p < 0.001).