Fig. 4: SD and SDA reverses the auranofin-induced phosphorylation of JNK in human neuroblastoma SH-SH5Y cells. | Cell Death & Disease

Fig. 4: SD and SDA reverses the auranofin-induced phosphorylation of JNK in human neuroblastoma SH-SH5Y cells.

From: Novel anti-apoptotic L-DOPA precursors SuperDopa and SuperDopamide as potential neuroprotective agents for halting/delaying progression of Parkinson’s disease

Fig. 4

A, B SH-SY5Y cells were incubated with 3 μM auranofin (Auf) for 30 min, washed, and treated with or without increasing concentrations of SD in 4 independent experiments. C, D SH-SY5Y cells were incubated with 3 μM auranofin (Auf) for 30 min, washed, and treated with or without increasing concentrations of SDA in 3 independent experiments, as indicated. Cell lysates proteins in equal amounts were separated on 10% SDS-PAGE, and analyzed by immunoblotting with the corresponding antibodies. The blots were cut prior to hybridization with antibodies shown in Fig. S8. The ratios of phosphorylated JNK to unphosphorylated JNK, or β-catenin were calculated. The values shown are averages (±SEM) based on four independent experiments, normalized to the phosphorylation state of cells treated with Auf after 3.5 h and plotted with a linear regression program. Student’s t-test (two populations) was performed for AuF treated cells. *P value < 0.05; **P value < 0.01; ***P value < 0.005.

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