Fig. 5: SD and SDA reverse Auf-induced p38MAPK phosphorylation in human neuroblastoma SH-SY5Y cells. | Cell Death & Disease

Fig. 5: SD and SDA reverse Auf-induced p38MAPK phosphorylation in human neuroblastoma SH-SY5Y cells.

From: Novel anti-apoptotic L-DOPA precursors SuperDopa and SuperDopamide as potential neuroprotective agents for halting/delaying progression of Parkinson’s disease

Fig. 5

A, B SH-SY5Y cells were incubated with 3 μM Auf for 30 min, washed, and treated with or without increasing concentrations of SD. C, D SH-SY5Y cells were incubated with 3 μM Auf for 30 min, washed, and treated with or without increasing concentrations of SDA for 3.5 h. Proteins in equal amounts of cell lysates were separated on 10% SDS-PAGE, and analyzed by immunoblotting with the corresponding antibodies. The blots were cut prior to hybridization with antibodies shown in Fig. S9. Phosphorylation of p38MAPK was quantified by immunoblot densitometry. The ratios of phosphorylated p38MAPK to the housekeeping β-catenin were calculated based on three independent experiments. The values shown are averages (±SEM) normalized to the phosphorylation state of cells treated with Auf after 3.5 h and plotted with a linear regression program. Student’s t-test (two populations) was performed for Auf treated cells. *P value < 0.05; **P value < 0.01; ***P value < 0.005.

Back to article page