Fig. 1: NR2F1-AS1 expression is increased in PC tissues and cell lines. | Cell Death & Disease

Fig. 1: NR2F1-AS1 expression is increased in PC tissues and cell lines.

From: Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis

Fig. 1

A Bioinformatics analysis of NR2F1-AS1 expression using TCGA-PAAD database on GEPIA (http://gepia2.cancer-pku.cn/#index) (T, tumor; N, normal). B, C Kaplan–Meier curves showing overall survival (OS) (B) and disease-free survival (DFS) (C) in PC patients stratified according to NR2F1-AS1 expression in TCGA-PAAD cohort. D qRT-PCR analysis of NR2F1-AS1 expression in 90-paired PC and matched adjacent tissues. E qRT-PCR analysis of NR2F1-AS1 expression in six PC cell lines and normal HPDE cells. F Analysis of NR2F1-AS1 expression in PC and non-tumor tissues by ISH; scale bar = 500 μm. G Survival analysis according to NR2F1-AS1 expression among 74 PC patients via Kaplan–Meier curves compared with the log-rank test. H FISH to detect the subcellular localization of NR2F1-AS1 (red) in MIA PaCa-2 and PANC-1 cells. Nuclei were counterstained with DAPI; scale bar = 10 μm. I qRT-PCR analysis of the localization and expression levels of NR2F1-AS1 in the cytoplasm and nucleus of MIA PaCa-2 and PANC-1 cells. Data are expressed as mean ± SD; *P < 0.05, **P < 0.01, ***P < 0.001.

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