Fig. 2: NR2F1-AS1 promotes the proliferation of PC cells in vitro. | Cell Death & Disease

Fig. 2: NR2F1-AS1 promotes the proliferation of PC cells in vitro.

From: Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis

Fig. 2

A CCK-8 assays were performed to examine the proliferation of NR2F1-AS1-depleted MIA PaCa-2 and PANC-1 cells. B Representative images of colony-formation assays in NR2F1-AS1-knockdown and control MIA PaCa-2 and PANC-1 cells. C EdU proliferation assays in NR2F1-AS1-knockdown and control MIA PaCa-2 and PANC-1 cells. D Wound-healing assays in NR2F1-AS1-knockdown and control MIA PaCa-2 and PANC-1 cells. E Transwell assays were used to evaluate the migration and invasion abilities of NR2F1-AS1-knockdown and control MIA PaCa-2 and PANC-1 cells. F Western blot analysis of epithelial-to-mesenchymal transition markers in NR2F1-AS1-knockdown and control PC cells. Scale bar = 200 μm. Data are expressed as mean ± SD. All experiments were repeated three times independently. *P < 0.05, **P < 0.01, ***P < 0.001.

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