Fig. 3: Silencing of NR2F1-AS1 inhibits tumor formation in nude mice. | Cell Death & Disease

Fig. 3: Silencing of NR2F1-AS1 inhibits tumor formation in nude mice.

From: Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis

Fig. 3

A Image of xenograft tumors resected from nude mice-carrying PANC-1 cells that transduced with lentiviruses encoding sh-Control, sh-NR2F1-AS1#1, or sh-NR2F1-AS1#2 (n = 5). B Growth curves of the subcutaneous tumor were made from weekly measurements of tumor volume. C Weights of the subcutaneous tumors in each group were measured after 5 weeks when the mice were sacrificed. D Representative images of IHC staining for Ki-67 and PCNA in the tumor sections from sh-Control, sh-NR2F1-AS1#1, or sh-NR2F1-AS1#2 xenografts; scale bar = 100 μm. E–H Representative fluorescent images of microscopic metastatic nodules after 5 weeks (n = 5), and liver (G) and lung (H) tissues stained with hematoxylin and eosin (H&E); scale bar = 20 μm. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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