Fig. 4: NR2F1 is a target of NR2F1-AS1 and is positively regulated by NR2F1-AS1. | Cell Death & Disease

Fig. 4: NR2F1 is a target of NR2F1-AS1 and is positively regulated by NR2F1-AS1.

From: Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis

Fig. 4

A Bioinformatics analysis of the correlation between the genomic locations of NR2F1-AS1 and NR2F1. B The expression level of NR2F1 in the TCGA-PAAD database (T, tumor; N, normal). C NR2F1 expression in PC and matched adjacent tissues (n = 90) was detected by qRT-PCR. D IHC analysis of NR2F1 expression in PC and adjacent tissues using tissue microarrays; scale bar = 500 μm. E, F qRT-PCR (E) and western blot (F) results showing NR2F1 expression in six PC cell lines compared with the HPDE cell line. G Correlation between NR2F1-AS1 and NR2F1 expression in PC tissues. H Correlation between NR2F1 and NR2F1-AS1 expression levels in the TCGA-PAAD dataset. I, J qRT-PCR (I) and western blot (J) results for NR2F1 expression in PANC-1 and MIA PaCa-2 cells after introducing NR2F1-AS1-knockdown constructs (sh-NR2F1-AS1#1 and sh-NR2F1-AS1#2) or the negative control (sh-Control) by lentiviral infection. Data are expressed as mean ± SD. *P < 0.05, **P < 0.01, ***P < 0.001.

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