Fig. 6: NR2F1-AS1/NR2F1 activates the AKT/mTOR pathway in PC cells. | Cell Death & Disease

Fig. 6: NR2F1-AS1/NR2F1 activates the AKT/mTOR pathway in PC cells.

From: Hypoxia-induced long noncoding RNA NR2F1-AS1 maintains pancreatic cancer proliferation, migration, and invasion by activating the NR2F1/AKT/mTOR axis

Fig. 6

A The pathway analysis of NR2F1-AS1 with co-expressed genes using COEXPREdb (http://coxpresdb.jp/) by the KEGG. B Western blot analysis of AKT, p-AKT, mTOR, p-mTOR, p70 S6K, and HIF-1α in the indicated PC cells. C CCK-8 assays in the NC, NR2F1-AS1, NR2F1-AS1 + siNR2F1, and NR2F1-AS1 + LY294002 groups of PANC-1 and MIA PaCa-2 cells. D Colony-formation assays in the indicated PC cells. E EdU assays in the indicated PC cells. F Transwell assays showing the migration and invasion abilities of the indicated PC cells. G Western blot analysis of NR2F1, AKT, p-AKT, mTOR, p-mTOR, p70 S6K, and HIF-1α in the indicated PC cells. Scale bar = 200 μm. Data are expressed as mean ± SD. All experiments were repeated three times independently. *P < 0.05, **P < 0.01, ***P < 0.001.

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