Fig. 8: NR2F1-AS1 plays an important role in the hypoxia-induced migration and invasion of PC cells.

A IF staining of epithelial-to-mesenchymal transition markers in the indicated cells; scale bar = 100 μm. B Western blot analysis of NR2F1, Vimentin, and E-cadherin expression in the indicated MIA PaCa-2 and PANC-1 cells. C Wound-healing assays were used to detect the migration ability of the indicated MIA PaCa-2 and PANC-1 cells. D Transwell assays were used to show the migration and invasion abilities of the indicated cells. For C and D, scale bar = 200 μm. Data are expressed as mean ± SD. All experiments were repeated three times independently. *P < 0.05, **P < 0.01, ***P < 0.001.