Fig. 3: Fbxo45 binds to a conserved motif in USP49 and NEK6 enhances the degradation of USP49. | Cell Death & Disease

Fig. 3: Fbxo45 binds to a conserved motif in USP49 and NEK6 enhances the degradation of USP49.

From: Fbxo45 facilitates pancreatic carcinoma progression by targeting USP49 for ubiquitination and degradation

Fig. 3

A Schematic of USP49 domains and its mutant construct. B IB analysis of WCLs derived from Panc-1 cells transfected with the indicated plasmids. C IB analysis of IPs and WCLs derived from Panc-1 cells transfected with the indicated constructs. Cells were treated with 10 μM MG132 for 6 h before harvesting. D IB analysis of ubiquitination products and WCLs derived from Panc-1 cells transfected with the indicated constructs. Cells were treated with 10 μM MG132 for 6 h before harvesting. E IB analysis of WCLs derived from Panc-1 cells transfected with NEK6 siRNAs. F IB analysis of WCLs derived from Panc-1 cells transfected with the indicated constructs. G IB analysis of ubiquitination products and WCLs derived from Panc-1 cells transfected with the indicated constructs. Cells were treated with 10 μM MG132 for 6 h before harvesting. H IB analysis of ubiquitination products and WCLs derived from Panc-1 cells transfected with the indicated constructs. Cells were treated with 10 μM MG132 for 6 h before harvesting.

Back to article page