Fig. 6: CMTM6 reduced the phosphorylation of RB and downregulates E2F1 transcription factors activity. | Cell Death & Disease

Fig. 6: CMTM6 reduced the phosphorylation of RB and downregulates E2F1 transcription factors activity.

From: CMTM6 inhibits tumor growth and reverses chemoresistance by preventing ubiquitination of p21 in hepatocellular carcinoma

Fig. 6

A The HuH-7 cells stably overexpressing CMTM6 were transfected with scramble or two independent p21 siRNAs. The expression of p21, pRB and total RB was detected by Western blot analysis. B The HepG2 cells with stable CMTM6 depletion were transfected with empty vectors or vectors expressing p21. The expression of p21, pRB and total RB was detected by Western blot analysis. C Dual-luciferase reporter assays revealing the transcriptional activity of E2F1 on the promoters of CDC6, PCNA and TK1 in the indicated cells. D qPCR analysis of the mRNA levels of the candidate downstream targets of E2F1 in the indicated cells. The error bars represent the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001.

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