Fig. 8: SLC25A5 was involved in mitochondrial metabolism and activity, while it exerted an effect on tumorigenesis via the MAPK signaling pathway. | Cell Death & Disease

Fig. 8: SLC25A5 was involved in mitochondrial metabolism and activity, while it exerted an effect on tumorigenesis via the MAPK signaling pathway.

From: An integrated bioinformatic investigation of mitochondrial solute carrier family 25 (SLC25) in colon cancer followed by preliminary validation of member 5 (SLC25A5) in tumorigenesis

Fig. 8

A The expression of ENO-1, IDI-1, and HMGCR was upregulated, while EBP and MVD were downregulated when SLC25A5 was overexpressed. B Decreased mitochondrial membrane potential was determined by JC-1 staining, suggesting the inhibition of mitochondrial activity. C GSEA hinted that high expression of SLC25A5 was negatively related to the KRAS signaling pathway. D Protein levels of MEK1/2, pMEK1/2, Erk1/2, and p-Erk1/2 were detected by western blotting. E Representative images of TUNEL staining in cells in the presence of the MAPK activator PDBu (0.1 μg for 24 h) or dimethyl sulfoxide (DMSO, 0.1‰) served as controls. with or without SLC25A5 overexpression. F Quantitative results of the TUNEL assay. G CCK8 assays for SLC25A5-overexpressing cells with or without 0.1 μg PDBu treatment. H Western blot analysis of MEK1/2, pMEK1/2, Erk1/2, and p-Erk1/2 in SLC25A5-overexpressing cells with or without 0.1 μg PDBu treatment. β-actin protein was used as the internal control. n = 3 biological replicates. Scale bar = 20 μm. Statistical analysis: Student’s t test. *p < 0.05, **p < 0.01, ***p < 0.001, ****p < 0.0001.

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