Fig. 2: STYX overexpression promotes GC cells proliferation and migration in vitro. | Cell Death & Disease

Fig. 2: STYX overexpression promotes GC cells proliferation and migration in vitro.

From: Pseudophosphatase STYX is induced by Helicobacter pylori and promotes gastric cancer progression by inhibiting FBXO31 function

Fig. 2

A Western blot analysis of STYX protein level in GC cells transfected with the control vector (pCMV) or STYX overexpression vector (STYX). B qRT-PCR analysis of STYX mRNA level in transfected GC cells. C EdU analysis of the cell proliferation ability in transfected GC cells. The representative results were showed. D Statistical analysis of the EdU-positive cell ratio in GC cells. The data are expressed as the means ± SD from six independent experiments. E CCK-8 analysis of the cell proliferation ability in transfected GC cells. The data are expressed as the means ± SD from three independent experiments. F Transwell migration assay in transfected GC cells. The representative results were showed. G Statistical analysis of the cell numbers passing through the transwell filter in GC cells. The data are expressed as the means ± SD from three independent experiments. The data shown in D and G were analyzed using GraphPad Prism v7.0 4 software with a nonpaired Student t-test. The data shown in E were analyzed using a two-way ANOVA test. **p < 0.01; ***p < 0.001; ****p < 0.0001.

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