Fig. 5: STYX interacts with FBXO31. | Cell Death & Disease

Fig. 5: STYX interacts with FBXO31.

From: Pseudophosphatase STYX is induced by Helicobacter pylori and promotes gastric cancer progression by inhibiting FBXO31 function

Fig. 5

A The interaction between STYX and FBXO31 was found through the analysis of Biogrid database (https://thebiogrid.org/). B, C Myc-FBXO31 or Flag-STYX were co-transfected into SGC-7901 cells for 48 h. Co-immunoprecipitation was performed with either Myc antibody to probe for STYX by anti-STYX antibody or Flag antibody to probe for FBXO31 by anti-Myc antibody. Immunoglobulin (IgG) was used as a control. D Endogenous interaction between FBXO31 and STYX protein was detected by co-immunoprecipitation with the FBXO31 or STYX antibody in 293 T cells and western blot were performed to detect the co-precipitated STYX or FBXO31. IgG was used as a control. E, F pCMV or Myc-FBXO31 or Myc-FBXO31ΔF and Flag-STYX were co-transfected into SGC-7901 cells for 48 h. Co-immunoprecipitation was performed with either Myc antibody to probe for STYX by anti-STYX antibody or Flag antibody to probe for FBXO31 by anti-Myc antibody.

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