Fig. 4: MiR-381-3p inhibited NFAT5 by directly targeting its 3’UTR. | Cell Death & Disease

Fig. 4: MiR-381-3p inhibited NFAT5 by directly targeting its 3’UTR.

From: Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5

Fig. 4

A, B DEGs between NC and Pi group were presented by heatmap and volcano plot. C The intersection of different online databases and DEGs was presented by Venn graph. D The binding sites of miR-381-3p and NFAT5 were predicted by TargetScan. E The combination of miR-381-3p and NFAT5 was verified by dual-luciferase reporter gene assay, *P < 0.05. F, G The protein and mRNA expression levels of NFAT5 in HA-VSMCs were detected after transfection of miR-381-3p mimics, *P < 0.05 compared with the mimics NC group; #P < 0.05 compared with the Pi + mimics NC group; &P < 0.05 compared with the Pi + mimics 50 nM group. H, I The protein and mRNA expression levels of NFAT5 in HA-VSMCs were detected under treatment of different exosomes, *P < 0.05 compared with the NC group; #P < 0.05 compared with the Pi group; $P < 0.05 compared with the Pi + Exo group.

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