Fig. 5: MiR-381-3p inhibited apoptosis and VC by targeting NFAT5. | Cell Death & Disease

Fig. 5: MiR-381-3p inhibited apoptosis and VC by targeting NFAT5.

From: Bone marrow mesenchymal stem cell-derived exosomal microRNA-381-3p alleviates vascular calcification in chronic kidney disease by targeting NFAT5

Fig. 5

A, B The mRNA and protein expression levels of NFAT5 were detected after transfected with oe-NFAT5 or (and) miR-381-3p mimics. C Expression of miR-381-3p was detected by RT-qPCR. D–F Alizarin Red S staining, Ca2+ content, and AKP activity were detected after transfected with oe-NFAT5 or (and) miR-381-3p mimics (scale bar = 100 μm). G Apoptosis was measured by flow cytometry. H The protein expression of BAX, BCL-2, and cleaved caspase-3 was detected after transfected with oe-NFAT5 or (and) miR-381-3p mimics. *P < 0.05 compared with the Pi + mimics NC + ve group; #P < 0.05 compared with the Pi + mimics NC + oe group; &P < 0.05 compared with the Pi + mimics + ve group.

Back to article page