Fig. 2: PRMT5 is responsible for TMAO-induced VCAM-1 expression.
From: PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells
VSMC were transfected with PRMT5 siRNA (siPRMT5) or PRMT5 cDNA (PRMT5 OE), then stimulated with TMAO (600 μM) for 24 h. A, B Representative blots and quantitative analysis of VCAM-1 and PRMT5. The protein expression level was normalized to β-actin. C Representative images obtained by fluorescence microscope show cell adhesion. Data shown are means ± SD, n = 6 per group from three independent experiments. ***P < 0.001 using a one-way ANOVA followed by Tukey’s post hoc test.
