Fig. 4: PRMT5 contributes to TMAO induced vascular inflammation in vivo.
From: PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells
SMC-PRMT5-KO or SMC-Cre mice were subjected to wire-induced carotid artery injury. TMAO (86 μmol) or saline was administered daily by intraperitoneal injection for 7 days. A Representative photomicrographs for VCAM-1 in carotid arteries at 7 days after wire injury. Scale bar, 50 μm. B Representative photomicrographs for CD68 in carotid arteries at 7 days after wire injury. Scale bar, 50 μm. C Representative blots and quantitative analysis of VCAM-1 and PRMT5. The protein expression level was normalized to β-actin. D Representative blots of arginine methylation of p65. Immunoprecipitated SYM10 was blotted with an antibody against p65. Data shown are means ± SD, n = 6 per group from three independent experiments. ***P < 0.001 using a one-way ANOVA followed by Tukey’s post hoc test.
