Fig. 5: NOX4 knockdown decreases H2O2 production in VSMC treated with TMAO.
From: PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells
A Representative blots and quantitative data of Nox1, Nox2 and Nox4 protein expression in TMAO (600 μM) treated VSMC at indicated periods. The protein expression level was normalized to β-actin. B, C VSMC were treated with scrambled siRNA, Nox1 or Nox4 siRNA, and then treated with TMAO (600 μM). B H2O2 and C O2•− levels were evaluated by DHE and Amplex Red probe, respectively. Data shown are means ± SD, n = 6 per group from three independent experiments. **P < 0.01, ***P < 0.001 using a one-way ANOVA followed by Tukey’s post hoc test.
