Fig. 6: Nox4 knockdown downregulates PRMT5 and VCAM-1 expression.
From: PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells
A VSMC were pretreated with N-acetyl-cysteine (NAC) and then stimulated with TMAO (600 μM) for 24 h. Representative blots and quantitative analysis of VCAM-1 and PRMT5. The protein expression level was normalized to β-actin. H2O2 stimulation served as positive control. B–D VSMC were treated with scrambled siRNA (siScr) or Nox4 siRNA (siNOX4) and then treated with TMAO (600 μM). B Representative blots and quantitative analysis of Nox4 and PRMT5. The protein expression level was normalized to β-actin. C Representative blots and quantitative data of VCAM-1 protein expression. The protein expression level was normalized to β-actin. H2O2 stimulation served as positive control. D Representative images obtained by fluorescence microscope show cell adhesion. Data shown are means ± SD, n = 6 per group from three independent experiments. **P < 0.01, ***P < 0.001 using a one-way ANOVA followed by Tukey’s post hoc test.
