Fig. 7: NF-YA is responsible for TMAO-induced PRMT5 expression.
From: PRMT5 critically mediates TMAO-induced inflammatory response in vascular smooth muscle cells
A VSMC were treated with TMAO (600 μM) for indicated periods. Representative blots and quantitative analysis of NF-YA. The protein expression level was normalized to β-actin. B VSMC were transfected with scrambled siRNA (siScr) or Nox4 siRNA (siNox4) and then stimulated with TMAO (600 μM) for 24 h. Representative blots and quantitative analysis of NF-YA. The protein expression level was normalized to β-actin. C VSMC were transfected with NF-YA siRNA (siNF-YA) or siScr and then stimulated with TMAO (600 μM) for 24 h. Representative blots and quantitative analysis of NF-YA and PRMT5. The protein expression level was normalized to β-actin. Data shown are means ± SD, n = 6 per group from three independent experiments. *P < 0.05, **P < 0.01, ***P < 0.001 using a one-way ANOVA followed by Tukey’s post hoc test.
