Fig. 2: Stimulation of complement triggers podocyte pyroptosis. | Cell Death & Disease

Fig. 2: Stimulation of complement triggers podocyte pyroptosis.

From: Complement induces podocyte pyroptosis in membranous nephropathy by mediating mitochondrial dysfunction

Fig. 2

Podocytes were treated with or without C3a (1 μM) or C5a (100 nM). The pyroptotic features of podocytes, including GSDMD cleavage, membrane integrity, LDH release, and PI uptake, were assessed. A The expression of pyroptosis-related mRNAs was measured by qRT–PCR. B, C The levels of pyroptosis-related proteins were determined by western blotting. D The cell membrane integrity and apoptosis of podocytes were assessed using a microplate reader. E C3a and C5a promoted the release rate of LDH. F, G Microscopic imaging of podocytes stained with Hoechst 33342 and propidium iodide (PI) (scale bar = 50 μm). The right panel represents the percentage of PI-positive podocytes (6 and 16 high-power fields were analyzed in group NC and group C3a respectively in (F); 8 and 11 high-power fields were analyzed in group NC and group C5a respectively in (G)). The data in (A), (D), and (E) represent the mean ± SEM of three independent experiments. *p < 0.05.

Back to article page