Fig. 3: Inhibition of pyroptosis reverses podocyte damage caused by complement stimulation. | Cell Death & Disease

Fig. 3: Inhibition of pyroptosis reverses podocyte damage caused by complement stimulation.

From: Complement induces podocyte pyroptosis in membranous nephropathy by mediating mitochondrial dysfunction

Fig. 3

Podocytes were treated with or without pyroptotic inhibitors under C3a or C5a stimulation conditions. The pyroptotic features of podocytes were determined to validate the reversal effect of pyroptotic inhibitors on complement-induced cell damage. A, B Release rate of LDH from podocytes. C Representative microfluorographs of PI in podocytes cultured with or without pyroptotic inhibitors. Arrows indicate podocytes that stained positive for PI (scale bar = 50 μm). Quantification of PI-positive cells is shown in the panels below (13–20 high-power fields were analyzed in each group in the left panel; 12–15 high-power fields were analyzed in each group in the right panel). The data in (A) and (B) represent the mean ± SEM of three independent experiments. *p < 0.05.

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