Fig. 5: Inhibition of ROS reverses complement-induced pyroptosis of podocytes. | Cell Death & Disease

Fig. 5: Inhibition of ROS reverses complement-induced pyroptosis of podocytes.

From: Complement induces podocyte pyroptosis in membranous nephropathy by mediating mitochondrial dysfunction

Fig. 5

An inhibitor of ROS, NAC, was used to demonstrate that the production of mitochondrial ROS mediated C3a- or C5a-induced pyroptosis. A Representative images of mitochondrial ROS detected using MitoSOX in cultured podocytes (scale bar = 20 μm). Quantification is shown in the right panel (11–18 high-power fields were analyzed in each group). B Production of mitochondrial ROS was detected using flow cytometry in cultured podocytes. Quantification is shown in the right panels. C Protein level of pyroptosis-related molecules in podocytes. D Release rate of LDH from podocytes. E Representative microfluorographs of PI staining in podocytes (scale bar = 50 μm). Quantification is shown in the right panel (11–17 high-power fields were analyzed in each group). The data in (B) represent the mean ± SEM of three independent experiments. *p < 0.05. The data in (D) represent the mean ± SEM of four independent experiments. *p < 0.05.

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