Fig. 3: Mitochondria-associated membranes (MAMs) purified and identified from rat heart and GA muscle. | Cell Death & Disease

Fig. 3: Mitochondria-associated membranes (MAMs) purified and identified from rat heart and GA muscle.

From: Ultrastructural and proteomic profiling of mitochondria-associated endoplasmic reticulum membranes reveal aging signatures in striated muscle

Fig. 3

A Scheme for mitochondria-associated membranes (MAMs) using Percoll gradient centrifugation. B Subcellular fractions prepared from rat heart were validated by transmission electronic microscopy (TEM). Bar = 0.5 μm. C Protein components of subcellular fractions prepared from rat heart and GA muscle were revealed by immunoblot analysis. CALR (calreticulin) and GAPDH were used as markers for ER and cytosolic fractions, respectively; IP3R and CANX (calnexin) was considered as markers of both ER and MAMs, whereas VDAC1 and GRP75 was adopted as markers of both mitochondria and MAMs. D and E The protein content (in mg) of MAMs, pure mitochondria and microsome was quantified and normalized by total tissue mass (in g) to obtain the relative protein abundance of subcellular fractions in heart (D) and GA muscle (E) (n = 6 rats per group, data represent mean ± SD. Variance was similar between groups being compared. *P < 0.05; **P < 0.01). PNS post-nuclear supernatant, Mc crude mitochondria, Mp pure mitochondria, Microsome microsome/ER fraction, MAM mitochondria-associated membrane, Cyto cytosol.

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