Fig. 5: Functional overview of MAM proteome in aged striated muscle.

A Enrichment map for the striated muscle derived-MAM proteome between 4-month-old vs. 24-month-old rats. Meta-analysis of the MAM proteome derived from the heart and GA muscle were combined using the Fisher’s method (–2*∑ log P < 0.05), and plotted with Cytoscape after Gene-Set Enrichment Analysis (GSEA). The enrichment map was created with parameters q < 0.1, and Jaccard Overlap combined coefficient >0.375 with combined constant = 0.5. Gene-set nodes were colored according to the Normalized Enrichment Score (NES): red represented enrichment in the 4-month-old group, whereas blue represented enrichment in the 24-month-old group. The number of proteins in each gene-set was proportional to their node size. Edge thickness was proportional to the overlap between gene-sets. All gene-sets have P < 0.05. B and C The GSEA enrichment plots showed the MAM-associated biological processes (B) and cellular components (C), which were significantly changed during aging in rat heart and GA muscle. The most enriched biological processes involved in metabolism, material transport and organelle organization were highlighted by purple, lime green and green in colors respectively, corresponding to the colors shown in A. (n = 3 per group, P and NES were calculated by GSEA, and only pathway with –log P > 1.5 were shown). Mito. mitochondrial, TCA tricarboxylic acid, Metab. metabolism, ETC electronic transmission chain, IMM inner mitochondrial membrane, OMM outer mitochondrial membrane.